Showing posts with label Using Microscope. Show all posts
Showing posts with label Using Microscope. Show all posts

Free article : Use Of The Microscope

September 21, 2010

The compound microscope is a precision optical instrument which has a normal life expectancy of over forty years if cared for properly. Today we hope assist you in learning to use the instrument correctly.

A. There are four general considerations one should learn before begining :

  1. Clean the lenses only with the lens tissue provided. Be sure that you do not remove any of the lenses.
  2. Attempt no repairs or adjustments, ask your instructor.
  3. Handle as carefully as you would an expensive camera. 
  4. At the close of the period you should : 
  • Know how to carry and clean the instrument
  • Be able to adjust the light correctly.
  • Be able to identify the : base, stage, diaphragm, tube, nosepiece, ocular, 10x (low power) objective, 43x (high power) objective.
  • Be able to locate an object readily and to focus the microscope correctly.
  • Be capable of determining the dimensions of an object.
  • Know how to convert milimeters (mm) to microns.  
B. Focusing the Microscope 
  1. Prepare your microscope for use by placing it in a comfortable working position not too near the edge of the table.
  2. Adjust the nosepiece so that the low power (10x) objective cliks into position over the hole in the stage.
  3. Adjust the diaphragm, using the largest hole (No. 4) in the disc.
  4. Raise the body tube so that the low power objective is about one (1) inc above the stage. Move the stage clips to one side. While observing from one side of the microscope, place the slide (with the side containing the coverslip up) on the stage of the microscope and adjust the slide so that the object to be studied is in the approximate center of hole in the stage.      :to be continue ......

Read more...

Using Objective Lens 100 times Direct

August 10, 2010

When studying or observing bacteria, more efficient to start with the oil immersion lens (100 x) and ignore the preliminary steps with objektif lens with low magnification power. This may by students who are current users / familiar and experienced using a microscope that can skip the use of objective lens and 10 x 40 x before using 100 x objective lens directly.

Tools and materials:
  • Preparations of representative microbes: bacteria, fungi, etc..
  • Microscope
  • lens paper
  • Immmersion oil

How it Works:
  1. Place the specimen on the slide in the middle of the skylight, just under the oil immersion lens will be placed.
  2. Add a drop of immersion oil on the specimen
  3. Ensure that there is enough distance between oil and 100 x objective lens before you rotate the lens over the specimen.
  4. Set konensor that its position is just below the specimen slide.  Close the condenser diaphragm almost entirely if you see live specimens, otherwise open the diaphragm condenser when viewing the stained specimen.
  5. By using makrometer, reduce the distance between the lens immersion oil with the specimen, until the lens is submerged in oil and almost touching the specimen. Working distance for the 100 x objective lens is approximately 0.1 mm.
  6. Now see it through a microscope and find the shadow of the specimen by increasing the distance between the specimen and the lens by turning the micrometer.
Move slides forward and back slowly when you are looking for focus. Moving shadow is often more easily seen than the silent shadows. If you do not find the shadow after 3-5 rounds or when the lens is lifted from the oil, repeat steps 5 and 6.

Reasons that are impossible if you do not find anything under the oil immersion lens is as follows:
  1. Too much immersion
  2. Moving too fast micrometer
  3. Condenser is not set correctly (usually a diaphragm iris too open or closed)
  4. Dirty lens
  5. Too few organisms on the slide
  6. Specimens are not attached to the middle of the field before turning the lens
  7. Slide upside down.

Read more...

Using Microscope

March 08, 2010



On the picture above
  1. Pick up microscope with both hands. One in the base, another in the arm.
  2. Rotate revolver until ‘click’ sound, so the objective with weakest magnification placed on the middle of objective stage (picture a). Set the mirror below until sunlight reflect into ocular (picture b).
  3. Put object glass right on middle of object stage. Set until the gap between object glass and stage get close or almost contact (± 0.5 cm).
  4. hile seeing the ocular, rotate the coarse adjustment knob to set the focus until object appear. Rotate the fine adjustment knob until object seen clearly. Shift the object if necessary.
  5. Use bigger magnification if necessary. Rotate the revolver to change lens.

Note: for strong magnification (1000x) use immersion oil. Pick up the object glass and drop the immersion oil on the object. Put dawn again the object glass on microscope and rotate the adjustment knob until objective lens contact with immersion oil. Vision seen in microscope is opposite current with the object. If the object glass shifted to left, the vision will shifted to right, just the opposite If the object glass shifted to right, the vision will shifted to left.

    Read more...

      © Blogger template The Professional Template II by Ourblogtemplates.com 2009

    Back to TOP  

    Search Engine Submission - AddMe