Microscope slide preparation technique

How to make microscope slide object preparation? Picture above is one of kind sample slide preparation made from complex process. There are many methods for preparation making. Plant tissue preparation is different with histology preparation in making method. On this post, I have concern more to histology and plant tissue preparation making.

Study about preparation making is known as micro technique (in this post, plant micro technique). This study is especially to learning about making long age slide preparation or fresh slide preparation. Paraffin method and wood slicing method are methods that will be learned in this post. In paraffin method, plant organ kept in paraffin and sliced into thin slices and colored before ready to observe in microscope.

Wood slicing method needs special treatment. Specific differences in every kinds of plant need us to knowing well about the life cycle of each plant. We need to know every reagent give effect to each kind of plants.

Making of plant slide preparation

Making of animal slide preparation

Read more...

Making of plant slide preparation

Sampling This step is to decide what kind of plant and organ or tissue that we need. Click here to see the process

Fixation Fixation is using FAA solution (formalin, alcohol, acetic acid glacialic) for at least 24 hours. Fixation purpose is to makes sample died and increase durability of sample without change the condition and appearance of the sample. But if we not do this process carefully, it can make sample damaged. To see fixation process, click here or here.

Aspration After fixation, we have to out air in plant tissue to avoid penetration of FAA solution blocked. We can use this tools and the process is like this.

Dehydration This step purposes is to out water in plant tissue so that tissue can exposed with paraffin. We usually do this process with alcohol-xylene solution or alcohol TBA solution. See each step of dehydration here.

Clearing Clearing purposes is to make sample colorless. See clearing step here.

Infiltration Infiltration is process to make paraffin can expose on the plant tissue. Infiltration process here.

Embedding On this step, we need container to make paraffin box or cubicle. Cubicle paraffin is used as place of plant tissue. Embedding process showed here

Paraffin slicing See the process here.

Patching We need totally clean of object glass to avoid loosing the slide. wipe up the object glass with dry and clen wiper. For patching agent, we can use Haupt’s solution. see the process here.

coloring To simplify this step, we can make coloring schedule. The simplest coloring method is progressive method when intensity of color in tissue is straight equivalent with time to submerged in coloring agent. For chart sample, here

Covering with glass cover

Observation

Read more...